effect of synthetically prepared mixed-metal polymer on experimentally induced tumour in the skeletal muscles and liver of adult female albino mice: histological and ultrastructural studies

Metals have played an important role in medicine for years. One of the main objectives of many studies is to develop new metal compounds with an anticancer activity. The present work was designed to study the effect of new conjugated polymer as an antitumour agent against experimentally induced Ehrlich tumour. Thirty nine adult female albino mice were used and divided into 3 groups, the first one was the control group of liver, the second was the mice bearing Ehrlich tumour and the third was inoculated with the Ehrlich tumour and was treated with tested compound. The specimens from liver and tumour were processed for light and electron microscopic examination. Moreover, statistical analysis was used for evaluation of tumour volume in different groups. Light microscopic examination revealed multiple nodules with hyperchromatic nuclei of different shapes and sizes scattered through out the muscle in untreated mice. The muscle tissue appeared as structurless acidophilic necrotic masses. In treated group, regression of size of nodules occurred with necrosis of muscle and cellular debris. The liver of untreated animals revealed hyperchromatic nuclei of different shape and size around central vein and portal venule. The liver of treated animals showed reduction of cellular infiltration with necrosis of hepatocytes. Ultrastructural study of untreated tumour cells revealed nuclear pleomorphism with hyperchromatic change, some nuclei showed necrotic change. After treatment, the tumour cells showed apoptotic change. Ultrastructural study of liver cells affected by tumour showed vacuolation of cytoplasm and abnormal mitochondria. The treated liver cells were highly vacuolated with dilated bile canaliculs. The fluorescence microscope revealed an increase in apoptotic tumour cells in treated group compared with untreated one. Moreover, there was a significant decrease in tumour volume in treated group compared with untreated one. The tested compound showed antitumour activity against Ehrlich tumour

Diferencias entre los ácidos ribonucleicos mensajeros de hígado normal y de hepatoma de Novikoff / Differences between the messenger ribonucleic acids of the normal liver and Novikoff's hepatoma

En la activación de varios genes en algunas células cancerosas aparentemente se requiere que se desencadene una cascada de eventos, que trae como consecuencia la transcripción de diversos genes; al mismo tiempo, otros genes son reprimidos. El propósito de este trabajo fue investigar si existen diferencias en la transcripción del ácido ribonucleico mensajero (mRNA) del hígado normal y del hepatoma de Novikoff. En todos los experimentos se utilizaron ratas macho adultas Sprague Dawley. La extracción del RNA total de bazo, hígado y células del hepatoma de Novikoff se llevó a cabo con el método de Schueltz y la separación del mRNA se realizó utilizando una columna de celulosa-ácido oligotimidílico, de acuerdo a lo señalado por Aviv y Leder. La cuantificación del mRNA mostró que las células del hepatoma de Novikoff tienen el doble de esta macromolécula comparadas con el hígado normal de rata

Surface Properties of Cell Membrane in Early Stage of Transformed Cell -I. Early Detection of Transformed Cell by Concanavalin A; II. Properties of Plasma Membrane of Transformed Rat Liver Cell Induced by 3'-Me DAB

The present study was designed in order to investigate the lectin induced cytoagglutination properties of normal and transformed cells and surface alterations in the early stage of the transformed cells by characterizing the structural changes on the hepatoma surface membrane. Rat and rabbit erythrocytes and Sarcoma 180 ascites tumor cells were used for the lectin-induced cytoagglutination. Plotting % agglutination versus concanavalin A(Con A) concentration, sigmoid curves appeared in all cases. alpha-methyl-D-mannopyranoside(alphaMM) inhibited Con A induced cytoagglutination and the degrees of inhibition depended on the cell types and species. When rats were fed a diet containing 0.06% 3'-methyl-4dimethylaminoazobenzene(3'-Me DAB) for 12 weeks, almost all of the rats had solid liver tumors. Polyacrylamide gel electrophoresis of surface membrane proteins of these rat livers and of transplanted tumor cells showed three distinct protein bands, of which two were absent in normal rat livers. The molecular weights of these proteins were 73,000, 66,000, and 57,000 daltons. Antiserum against primary hepatocarcinoma surface proteins precipitated with three membrane proteins obtained from primary hepatocarcinoma cells as well as transplanted hepatocarcinoma cells, suggesting the presence of specific tumor antigens in these cells.